Disabled parking bays are marked and reserved on flat, hard surfaces. It is recommended to contact staff ahead of time with specific requirements. The Cellar Door and Tasting Room entrances are step-free, with the Tasting Room offering an accessible toilet featuring a wider door, emergency pull cord, and grab rails. Winery tours include access to the lower ground floor via a lift or stairs, although the vineyard portion is on gently sloped grass which may be uneven. The Flint Barns accommodation has a ramp available upon request for its entrance, and all ground-floor communal areas and en suite showers are level however, there is no lift to upper floors, and The Cottage accommodation is not wheelchair-accessible. The Estate Office is accessible via a permanent ramp and includes a fully accessible toilet.

While vineyard tours are not suitable for mobility aids or wheelchairs due to uneven ground, wine tastings held on the gravelled terrace or in the tasting room are accessible for guests using wheelchairs or other mobility aids. Accessible toilets are also available. No information is provided regarding accessible car parking or free entry for carers.

Sedlescombe Organic Vineyard offers good accessibility, including parking areas for disabled visitors and ramp/level access. While tours involve walking through the vines, general disabled access is provided, and the staff are available to assist those who require it. Guide dogs are permitted. It is advisable to contact the vineyard if specific assistance is needed, especially for tours that require booking in advance.

While the vineyard itself is uneven, areas for the vineyard tour and tasting are generally wheelchair accessible and suitable for all physical fitness levels. The Vineyard Hollow accommodation is on one floor and accessible for wheelchair users, though its path has low steps and the wet room bathroom does not have rails. Accessible parking is available by the winery.